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Premier Logo Created with Sketch. Eight pools of dextramers were constructed with each dextramer present in 4 of the 8 pools see Fig 1B for dextramer assignments. One may conclude that TCRa is not relevant for antigen specificity. Comparison with antigen-specific clonotypes identified in the literature We also queried the CDR3 protein sequences from the antigen-specific clonotypes identified with peptide-based MIRA against a set of published CDR3 amino acid sequences. The sum frequency of all clonotypes specific for a given dextramer within an individual also provided a quantitative measure of the response and varied depending on both the dextramer and individual Fig 2 ; S1 Table. Unconjugated antibodies directed against CD28 and CD49d were then added to the wells containing the suspended cells. Generation of cytomegalovirus-specific human T-lymphocyte clones by using autologous B-lymphoblastoid cells with stable expression of pp65 or IE1 proteins:
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